"No way to bring anything into Aleppo", as US says it will formally probe Syrian civilian deaths in coalition strikes.
The Syrian army says that pro-government forces have cut off all supply routes into the eastern, rebel-held part of Aleppo in the country's north.
According to the UK-based Syrian Observatory for Human Rights, eastern Aleppo has been under effective siege since July 11, and advances in recent days by forces loyal to President Bashar al-Assad have strengthened their control of the only route in.
"Today there is no way at all to bring anything into Aleppo," Observatory Director Rami Abdel Rahman said on Wednesday.
Once Syria's largest city, Aleppo has been roughly divided between government control in the west and rebel control in the east since mid-2012.
Taking full control of the city would be a significant victory for Assad.
An advance by pro-government forces around the only remaining supply route into the eastern sector this month enabled them to fire on it at close range, making the battlefront Castello road too deadly to use and putting at least 250,000 people in rebel-held districts under siege.
On Tuesday, the general command of the Syrian army called on opposition fighters in eastern Aleppo to lay down their weapons, the government-run SANA news agency reported.
In text messages to civilians and fighters, the army urged residents to "join the national reconciliations and expel the mercenary outsiders from the areas where the citizens reside", SANA added.
State media also said humanitarian corridors have been created for residents who wantes to escape the besieged areas of the city.
"Opposition activists are saying they are facing more dire circumstances - that the siege is creating a humanitarian crisis in and around the city," said Jamjoom.
In the last few days, dozens of Syrians have been reportedly killed in separate attacks across the divided city.
On Tuesday, Syrian government helicopters targeted the al-Mashhad neighbourhood in the rebel-held half of Aleppo, killing at least 18 people, according to the Observatory.
On Monday, more than 42 civilians were reported to have been killed and dozens more wounded in air strikes launched by the Syrian government in al-Atareb, a city in Aleppo province in the country's northwest.
The latest attacks came as the United Nations said it hoped to restart stalled peace talks in August.
Previous attempts at a diplomatic solution to end Syria's five-year-old civil war collapsed in April, partly due to an uptick in violence in Aleppo.
In separate events, the US army said on Wednesday it had enough evidence to start a formal investigation into allegations US-led coalition air strikes killed civilians near Manbij, a city in the Aleppo governate.
After examining "internal and external information," the coalition determined that there was sufficient credible evidence of civilian victims to open a formal inquiry, said spokesman Colonel Chris Garver.
According to witness reports, between 56 and 167 civilians died in attacks north of Manbij on July 19.
The US has been conducting military activity in Syria as part of an international coalition set up to battle the Islamic State of Iraq and the Levant (ISIL, also known as ISIS) armed group
The US-led coalition has been supporting mostly Kurdish opposition forces in and around Manbij since May, in the hope of breaking ISIL's hold on the region.
The Syrian conflict began as a mostly unarmed uprising against Assad in March 2011, but it quickly escalated into a full-blown civil war between the government, armed opposition fighters and hardline groups such as the Islamic State
The Observatory estimates that more than 280,000 people have been killed throughout the five years of bloodshed. Efforts to negotiate a lasting ceasefire have collapsed time and again.
More than 4.8 million Syrians have become refugees displaced from their homeland, while more than 6.5 million people are internally displaced within the country's borders.
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